
Laurence Cheng's MSc Defence
Poor efficiency of dietary fibre utilization limits pork production profit margins and environmental sus-tainability. This thesis research was to investigate stability of two processive endoglucanases, referred to as GH5-tCel5A1 and GH5-p4818Cel5_2A and overexpressed in the ClearColi® BL21 (DE3) cell. Three-dimensional models predicted presence of cysteine residues on the cellulases’ catalytic sites; and results of in vitro time course experiments further shown these cellulases were susceptible to auto-oxidation by air-borne O2 and were unstable.